Vol 37(2021) N 6 p. 101-110; DOI 10.21519/0234-2758-2021-37-6-101-110
A.V. Gildebrant1*, I.S. Sazykin1, and M.A. Sazykina1

Formation of Biofilms by Natural Microbial Strains in the Presence of Naphtalene and Anthracene

1Academy of Biology and Biotechnology named after D. I. Ivanovsky, Southern Federal University, Rostov-on-Don, 344090, Russia

Received - 16.04.2021; Accepted - 10.08.2021


In this work, the effect of low molecular polycyclic aromatic hydrocarbons (PAHs), naphthalene and anthracene, on the formation of biofilms by the strains of Acinetobacter calcoaceticus VKPM B-10353, Pseudomonas putida, and Vibrio aquamarinus VKPM B-11245 has been studied. The intensity of biofilm formation (staining with crystal violet), the number of viable cells (staining with fluorescein diacetate), as well as the metabolic activity of microbial cells included in biofilms (XTT-assay) were assessed. It was found that the studied strains are characterized by different responses to the introduction of pollutants. The V. aquamarines VKPM B-11245 strain showed mainly a protective reaction; in the presence of the studied PAHs an increase in the total bacterial biofilm biomass was observed, as well as a decrease in the number of living cells and the level of cellular metabolism. The naphthalene concentrations of 0.1% and 0.2% caused a reduction of all studied indicators. The P. putida strain is the most resistant to the action of the examined PAHs: in the presence of naphthalene, an increase in both the total biofilm biomass and cellular metabolism was observed. Anthracene enhanced cellular metabolism in this strain, which allows considering the studied P. putida strain as potentially useful for bioremediation. The A. calcoaceticus VKPM B-10353 strain was highly sensitive to naphthalene; suppression of biofilm formation was observed, as well as a decrease in cellular metabolism at all studied concentrations. However, anthracene increased biofilm biomass and enhanced the metabolism, which may indicate a potential ability of this strain to degrade this PAH.

Acinetobacter calcoaceticus, Pseudomonas putida, Vibrio aquamarinus, biofilm, anthracene, naphthalene

The research was financially supported by the Ministry of Science and Higher Education of the Russian Federation within the framework of state assignment no. 0852-2020-0029 in the field of scientific activity.