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Vol 37(2021) N 6 p. 84-94; DOI 10.21519/0234-2758-2021-37-6-84-94
A.N. Piniaeva1*, А.А. Kovpak1, Y.Y. Ivin1, А.А. Shishova1,2, A.A. Sorokin3, М.А. Prostova4, A.V. Belyakova1, А.А. Siniugina1, А.А. Ishmukhametov1,2, Y.H. Hapchaev1, А.P. Gmyl1

Selection of Sorbent for Poliovirus Vaccine Strain Concentrate Purification by Gel Filtration

1Chumakov Federal Scientific Center for Research and Development of Immune-and Biological Products, Russian Academy of Sciences, Moscow, 108819, Russia
2I.M. Sechenov First Moscow State Medical University, Moscow, 119991, Russia
3University of Lethbridge, Lethbridge, 4401 Canada
4Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

*pinyaeva_an@chumakovs.su
Received - 07.05.2021; Accepted - 30.09.2021

References

In the production of anti-virus vaccines, the replacement of wild-type with attenuated strains is becoming relevant. Sabin strains are the most suitable candidates for this replacement in the production of inactivated poliovirus vaccines. The main problem in the development of methods for purification of viral particles of Sabin strains is the preservation of their stability and antigenic properties. We have compared the sorbents used for gel-filtration of poliovirus concentrates and have selected a chromatographic resin, Sephacryl S-300HR, which provides a high yield of the target product and its high purity both during cultivation in roller bottles and in bioreactors. It was also shown that, along with effective purification, Sephacryl S-300HR allows preservation of the polyovirus antigenic properties and can be used in the preparation of monovalent inactivated poliovirus concentrates that meet the good quality requirements.

inactivated polio vaccine (IPV), oral polio vaccine (OPV), poliomyelitis, chromatographic purification, gel filtration

The authors grateful to L.P. Antonova and A.A. Butusova for their assistance in conducting the research.



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